ras related protein rab7  (Cytoskeleton Inc)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Effects of resistance training on alleviating hypoxia‐induced muscle atrophy: Focus on acetylation of FoxO1

doi: 10.1111/jcmm.18096

Figure Lengend Snippet: The levels of ALP and UPS in EDL of rats after the intervention ( n = 3) (two‐way ANOVA). The proteins expression of Rab7 (A1), Atg7 (A2), p62 (B2), Atrogin‐1 (C1), MuRF1 (C2) and Ub (C3) and the ratio of LC3II/I (B1). the IOD of Ac‐FoxO1 (D1) and the protein expression (D2) of Atg7. *Significant different from Group C; # Significant different from Group H ( p < 0.05).

Article Snippet: The target proteins were blocked with Odyssey Blocking buffer (LI‐COR, USA) and probed overnight at 4°C by using the antibodies of SIRT2 (Cat. No. ab211033, Abcam, UK), Ras‐related GTP‐binding protein 7 (Rab7) (Cat. No. ab137029, Abcam, UK), Atg7 (Cat. No. ab133528, Abcam, UK), microtubule‐associated protein light chain 3 (LC3) (Cat. No. NB100‐2220, Novusbio, USA), p62/SQSTM1 (Cat. No. ab109012, Abcam, UK), Atrogin‐1 (Cat. No. ab74023, Abcam, UK), MuRF1 (Cat. No. ab172479, Abcam, UK), Ubiquitin (Cat. No. sc‐8017, Santa cruz, USA), TATA binding protein (TBP) (Cat. No. 66166‐1‐Ig, Proteintech, China) and α‐tubulin (Cat. No. T6074, Sigma, Germany).

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Effects of resistance training on alleviating hypoxia‐induced muscle atrophy: Focus on acetylation of FoxO1

doi: 10.1111/jcmm.18096

Figure Lengend Snippet: The effect of FoxO1 acetylation on L6 myotube diameter and autophagy level ( n = 3) (Independent sample t‐test was used for comparison between normoxia and hypoxia group, and one‐way ANOVA was used for comparison between different plasmid groups in normal/hypoxia group). FoxO1 overexpression and Lysine mutant plasmid (simulated to maintain acetylation and deacetylation state) (A). Plasmids have been successfully transfected into the myotube (B). Myosin IF staining in normoxia (C1) and hypoxia (C2). Myosin is red, DAPI‐stained nuclei is blue, fluorescent marker is green. The myotube diameter (C3). The proteins expression of Rab7 (D1), Atg7 (D2), p62 (E2) and the ratio of LC3II/I (E1). a Significant different from Group EGFP in normoxia; b Significant different from Group EGFP in hypoxia; c Significant different from Group K to Q in normoxia; d Significant different from Group K to Q in hypoxia; e Significant different from Group rFoxO1 in hypoxia ( p < 0.05).

Article Snippet: The target proteins were blocked with Odyssey Blocking buffer (LI‐COR, USA) and probed overnight at 4°C by using the antibodies of SIRT2 (Cat. No. ab211033, Abcam, UK), Ras‐related GTP‐binding protein 7 (Rab7) (Cat. No. ab137029, Abcam, UK), Atg7 (Cat. No. ab133528, Abcam, UK), microtubule‐associated protein light chain 3 (LC3) (Cat. No. NB100‐2220, Novusbio, USA), p62/SQSTM1 (Cat. No. ab109012, Abcam, UK), Atrogin‐1 (Cat. No. ab74023, Abcam, UK), MuRF1 (Cat. No. ab172479, Abcam, UK), Ubiquitin (Cat. No. sc‐8017, Santa cruz, USA), TATA binding protein (TBP) (Cat. No. 66166‐1‐Ig, Proteintech, China) and α‐tubulin (Cat. No. T6074, Sigma, Germany).

Techniques: Comparison, Plasmid Preparation, Over Expression, Mutagenesis, Transfection, Staining, Marker, Expressing

Journal: Aging (Albany NY)

Article Title: LncRNA PVT1 promotes exosome secretion through YKT6, RAB7, and VAMP3 in pancreatic cancer

doi: 10.18632/aging.103268

Figure Lengend Snippet: PVT1 and exosome secretion-associated factors are increased in PC cell lines. ( A ) The expression of PVT1 in PC cell lines. ( B ) The mRNA expression of YKT6 in PC cell lines. ( C ) The expression of RAB7 in PC cell lines. ( D ) The expression of VAMP3 in PC cell lines. * P < 0.05, data are expressed as the mean ± SD.

Article Snippet: The primary antibodies used were as following: ras-related protein Rab-7 (RAB7) (1:100), CD63 (1:200), YKT6 v-SNARE homolog (YKT6) (1:100), and vesicle-associated membrane protein 3 (VAMP3) (1:100) (Applied Biological Materials Inc., Richmond, BC, Canada).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: LncRNA PVT1 promotes exosome secretion through YKT6, RAB7, and VAMP3 in pancreatic cancer

doi: 10.18632/aging.103268

Figure Lengend Snippet: PVT1 affects the expression and localization of RAB7 in HS766T cells. ( A ) The mRNA expression of Rab GTPases genes in PVT1-overexpressing HS766T cells. ( B ) The protein expression of RAB7 in PVT1-overexpressing HS766T cells. ( C ) Analysis of RAB7 (green) and CD63 (red) in PVT1-overexpressing HS766T cells, as determined by confocal microscope. ( D ) The interaction between PVT1 and RAB7, as determined by RIP assay and qRT-PCR. ( E ) The correlation between PVT1 and RAB7, as determined by pull-down assay. ( F ) The knockdown efficiency of si-RAB7 in HS766T cells. ( G ) The concentration of exosome derived from HS766T cells with overexpression of PVT1 and knockdown of RAB7. * P < 0.05, data are expressed as the mean ± SD.

Article Snippet: The primary antibodies used were as following: ras-related protein Rab-7 (RAB7) (1:100), CD63 (1:200), YKT6 v-SNARE homolog (YKT6) (1:100), and vesicle-associated membrane protein 3 (VAMP3) (1:100) (Applied Biological Materials Inc., Richmond, BC, Canada).

Techniques: Expressing, Microscopy, Quantitative RT-PCR, Pull Down Assay, Concentration Assay, Derivative Assay, Over Expression

Journal: Aging (Albany NY)

Article Title: LncRNA PVT1 promotes exosome secretion through YKT6, RAB7, and VAMP3 in pancreatic cancer

doi: 10.18632/aging.103268

Figure Lengend Snippet: Primer information.

Article Snippet: The primary antibodies used were as following: ras-related protein Rab-7 (RAB7) (1:100), CD63 (1:200), YKT6 v-SNARE homolog (YKT6) (1:100), and vesicle-associated membrane protein 3 (VAMP3) (1:100) (Applied Biological Materials Inc., Richmond, BC, Canada).

Techniques: Sequencing